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Image Search Results
Journal: British Journal of Pharmacology
Article Title: A novel and potent brain penetrant inhibitor of extracellular vesicle release
doi: 10.1111/bph.14789
Figure Lengend Snippet: Inhibition of EV secretion by PDDC and 5 in glial cells. Primary astrocytes were treated in parallel incubations with PDDC, 5, or cambinol in the 0.03–30 μM range as indicated; DMSO (0.02%) was used as vehicle control (set as 100% on y‐axis). Media was collected after 2‐hr incubation and centrifuged at 2,700 g for 15 min at 4°C. Supernatant was collected and the number of extracellular vesicles (EVs) was quantified using ZetaView Nanoparticle Tracker. The data points correspond to per cent inhibition of EV release (±SEM) over a concentration range for each compound; 100% release corresponds to 4.87 × 107 ± 0.098 EVs. Results are the average of three or four independent assays. Each independent assay was the average of three replicates. pEC50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] against response, using GraphPad Prism 7
Article Snippet: Extra cellular vesicles were quantified using
Techniques: Inhibition, Incubation, Concentration Assay
Journal: British Journal of Pharmacology
Article Title: A novel and potent brain penetrant inhibitor of extracellular vesicle release
doi: 10.1111/bph.14789
Figure Lengend Snippet: Inhibition of EV secretion by PDDC and 5 in glial cells. Primary astrocytes were treated in parallel incubations with PDDC, 5, or cambinol in the 0.03–30 μM range as indicated; DMSO (0.02%) was used as vehicle control (set as 100% on y‐axis). Media was collected after 2‐hr incubation and centrifuged at 2,700 g for 15 min at 4°C. Supernatant was collected and the number of extracellular vesicles (EVs) was quantified using ZetaView Nanoparticle Tracker. The data points correspond to per cent inhibition of EV release (±SEM) over a concentration range for each compound; 100% release corresponds to 4.87 × 107 ± 0.098 EVs. Results are the average of three or four independent assays. Each independent assay was the average of three replicates. pEC50 values and corresponding 95% confidence asymmetrical intervals, CI (95%), were obtained from a non‐linear fit of log [inhibitor] against response, using GraphPad Prism 7
Article Snippet: Extra cellular vesicles were quantified using ZetaView Nanoparticle tracker (
Techniques: Inhibition, Incubation, Concentration Assay